CD3 Antikörper
Kurzübersicht für CD3 Antikörper (ABIN490154)
Target
Alle CD3 Antikörper anzeigenReaktivität
Wirt
Klonalität
Konjugat
Applikation
Klon
-
-
Spezifität
- The mouse monoclonal antibody APA1/1 recognizes an activation-dependent intracellular epitope of CD3 epsilon. Exposure of the epitope precedes CD3 phosphorylation and recruitment and activation of ZAP70, which initiates the signaling cascade produced by T-cell activation. APA1/1 provides the earliest known marker for TCR-mediated T cell activation.
-
Aufreinigung
- Protein A purified
-
Immunogen
-
Purified human CD3 proteins isolated from thymus.
Type of Immunogen: Purified protein -
Isotyp
- IgG1
-
-
-
-
Applikationshinweise
-
Approved: Flo, ICC, IHC, IHC-Fr, IP, WB
Usage: Flow Cytometry: Positive control - Human T cells stimulated with anti-CD3 (MEM-57) antibody (1 μg/mL). Sample preparation:. At the end of stimulation of T cells, perform staining of surface markers (if required) in PBS + 0.1 % BSA for 20 min. on ice. Wash -
Kommentare
-
Target Species of Antibody: Human
-
Beschränkungen
- Nur für Forschungszwecke einsetzbar
-
-
-
Format
- Liquid
-
Konzentration
- Lot specific
-
Buffer
- PBS, 15 mM sodium azide, pH 7.4
-
Konservierungsmittel
- Sodium azide
-
Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Handhabung
- avoid freeze thaw cycles.
-
Lagerung
- 4 °C,-20 °C
-
Informationen zur Lagerung
- Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.
-
-
- CD3
-
Andere Bezeichnung
- CD3
-
Pathways
- T-Zell Rezeptor Signalweg, Ubiquitin Proteasome Pathway
Target
-